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Image Search Results
Journal: PLoS ONE
Article Title: Twist1 Controls Lung Vascular Permeability and Endotoxin-Induced Pulmonary Edema by Altering Tie2 Expression
doi: 10.1371/journal.pone.0073407
Figure Lengend Snippet: A ) Immunofluorescence micrographs showing cell-cell junction structure by VE-cadherin staining in L-HMVE cells treated with Twist1 siRNA #1 or #2 (bar, 50 µm). DAPI staining shows the nuclei of cells. Arrows show the region where cell-cell junctions are disrupted. As a control, cells were treated with siRNA duplex with an irrelevant sequence. B ) Graph showing the quantitation of the total discontinuous area of at least 10 fields (*, p<0.01). C ) Graph showing endothelial permeability in L-HMVE cells treated with control siRNA, Twist1 siRNA #1 or #2 (*, p<0.05). Permeability (Pa) values were evaluated after 6 h and are expressed as percentage of control cells. D ) Immunoblots showing tyrosine phosphorylated Tie2 detected by phosphotyrosine antibody (4G10) and total Tie2 immunoprecipitated with Tie2 antibody from total cell lysates in Twist1 knockdown (siRNA #1 treated) L-HMVE cells. E ) Levels of active RhoA in Twist1 knockdown (siRNA #1 treated) L-HMVE cells. Quantitative results (ratio of active RhoA to total RhoA) were normalized to control siRNA treated cells (*, p<0.01). F ) Graph showing endothelial permeability in HUVE cells treated with Twist1 siRNA #1, Tie2 DNA, or in combination (*, p<0.05). As a control, cells were treated with siRNA duplex with an irrelevant sequence and/or control DNA (vector only). Immunoblots showing Twist1, Tie2, and GAPDH protein levels in HUVE cells treated with Twist1 siRNA #1, Tie2 DNA, or in combination. All error bars are s.e.m.
Article Snippet: L-HMVE cells and
Techniques: Immunofluorescence, Staining, Sequencing, Quantitation Assay, Permeability, Western Blot, Immunoprecipitation, Plasmid Preparation
Journal: Advanced Pharmaceutical Bulletin
Article Title: The Role of Estrogen in Brain MicroRNAs Regulation
doi: 10.34172/apb.39216
Figure Lengend Snippet: The most abundant miRNAs in the brain
Article Snippet: miRNA-210 , The Notch signaling pathway up-regulation can be activated by miR-210 which contributes to angiogenesis after cerebral ischemia. miR-210 overexpression induces angiogenesis and neurogenesis. ,
Techniques: Functional Assay, Inhibition, Expressing, Over Expression, Cell Culture, Transgenic Assay, Migration